
Research Methods
Biological vs. Technical Replicates: What n Counts
Biological replicates measure biologically distinct samples; technical replicates repeat measurements of the same sample.
A result makes more sense once you know where the experiment happened and what the model could represent. Here we read methods as carefully as headlines, separating observations made in a dish, a living system, and a clinical setting.

Biological replicates measure biologically distinct samples; technical replicates repeat measurements of the same sample.

In vitro research is performed in a laboratory outside the body, such as work with cultured cells or a cell-free reaction.

In a laboratory assay, a positive control has an expected detectable response; a negative control has an expected absence of the target response or a…